<form id="thfbv"></form>


    <address id="thfbv"></address>
    <dfn id="thfbv"><listing id="thfbv"></listing></dfn>
    <address id="thfbv"><nobr id="thfbv"><meter id="thfbv"></meter></nobr></address>
    <noframes id="thfbv">
    
<address id="thfbv"><listing id="thfbv"><meter id="thfbv"></meter></listing></address>
      <address id="thfbv"></address>
      <sub id="thfbv"><address id="thfbv"><listing id="thfbv"></listing></address></sub>
      
    
      

      


      


        
      
	
        


      
    

      






      
    
    
      
        
      

            













      
    
      
        
      
            
      

                
                
      
                    
      
                    
      MicroVue Serum PYD EIA
      
                    
      貨號：8019
      
                    
      品牌：?QuidelOrtho
      
                    
      規格：96 Test
      
                    
      
                        目錄價：詢價 
                    
      
                    


                    
      
                        
      
                            
      
                                
                                
                                    
      
                                        
      
                                            
      
                                                
                                            
      
                                        
      
                                    
      
                                      		
                                
                            
      
                        
      
                    
      

                
      
            
      
        
      
    
      

      

      
    
    
      
        商品詳情
        相關下載
    
      
    
      

        
        
      
            
      
	For Research Use Only in the United States.
      
Not for use in diagnostic procedures.

      

      
	MicroVue Serum PYD provides a quantitative measure of the excretion of pyridinoline cross-link (Pyd) in serum. Pyridinoline cross-links are an indicator of type I collagen resorption, especially bone collagen.

      

      
	
      
		In humans, the total pool of urinary Pyd is approximately 45% free while the remaining fraction is bound to oligopeptides ranging from small linear peptides to very large cross-linked structures in excess of 10,000 Da. The proportion of free to total cross-links appears to be constant in healthy individuals and those with metabolic bone diseases or arthritis conditions, thus providing the rationale for measuring free Pyd.
	
      

      

      
	
      
		Performance Characteristics
	
      
	
      
		
      
			
      
				
      
					
      
						Method: Competitive
      
Analyte: Pyridinoline
      
Specimen: Serum
      
Specimen Volume: 25 μL post-filtration
      
LOD*: 0.4 nmol/L
      
LLOQ*: 0.75 nmol/L
      
HLOQ*: 12 nmol/L
      
Precision CVs within-run: 6-15%
      
Precision CVs between-run: 9-12%
      
Incubation Time: 20-26 Hours
      
Cross-reactivity: Free PYD 100% Free DPD 0% PYD/DPD peptides < 2.5% L-arginine 0.0004% (see Package Insert)
					
      
					
      
						*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification
					
      
				
      
			
      
		
      
	
      

      

      
	
      
		About the Assay
	
      
	
      
		
      
			
      
				
      
					
      
						Format
					
      
					
      
						96 well microtiter plate, with reagents sufficient to test 40 samples in duplicate
					
      
					
      
						Antibody Specificity
					
      
					
      
						Free pyridinoline cross-links, some cross-reactivity to glutamine and arginine (0.5 nmol/L signal)
					
      
					
      
						Species Reactivity
					
      
					
      
						Baboon, Cat, Cow, Dog, Guinea Pig, Horse, Human, Rhesus Macaque, Mouse, Rat 
      
No reactivity detected with Rabbit
					
      
					
      
						Assay Steps
					
      
					
      
						Spin serum in Spinfilters (included) for 30 minutes
      
Add 50 μL Reagent 1 to plate
      
Add 25 μL serum, diluted Standards, diluted Controls
      
Add 75 μL cold Pyridinoline Antibody
      
Incubate overnight at 2°C to 8°C
      
Wash 3 times with 1x Wash Buffer
      
Add 150 μL Enzyme Conjugate
      
Incubate 1 hour at room temperature
      
Wash 3 times with 1x Wash Buffer
      
Add 150 μL working Substrate Solution
      
Incubate 40 +/– 5 minutes at room temperature
      
Add 100 μL Stop Solution
      
Read at 405 nm
      
Data Reduction: 4 Parameter
					
      
				
      
			
      
		
      
	
      

      
        
      
        
        
    
      

    

      





        
      
        
    
      

      



      
    
      
        
    
      
    
      
        
    
      
    
      
        
    
      

      


      
    

      




      
        
        
        
        
    

      
    

69一级毛片